Screening of Tpo Gene C.1708c
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Abstract
Thirty seven patients (hypothyroidism, n=20 and thyroid cancer, n=17) and twenty five healthy individuals (control) were used in this study. These patients, who attended the endocrinologist in Nuclear Medicine Hospital and Al Yarmouk Nuclear Medicine Department in Baghdad, were selected during a period from July to October 2009.
Modified specific locked nucleic acid (LNA) primers were used for the detection of TPO gene mutations which include c.1708 C> T and c.1978 C>G. Seven mutations were found in TPO gene. Mutations involved transition cytosine by thymine at position 1708 of the exon 10 (c.1708C<T) were found in four thyroid cancer patients, while, transversion of cytosine by guanine at the position 1978 of exon 11 (c.1978C<G) were found in one thyroid cancer and two hypothyroidism patients. It can be concluded that the c.1708C<T and / or c.1978C<G mutant TPO in hypothyroidism and thyroid cancer patients studied are very likely to be properly expressed but lacks enzymatic activity.
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